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Association between three TLS genes and the tumor microenvironment. ( A – D ) Annotations of all cell types in the CRC_EMTAB8107 dataset. Proportions and expression levels of ( E ) <t>CCL2,</t> ( F ) PDCD1, and ( G ) ICOS.
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Association between three TLS genes and the tumor microenvironment. ( A – D ) Annotations of all cell types in the CRC_EMTAB8107 dataset. Proportions and expression levels of ( E ) CCL2, ( F ) PDCD1, and ( G ) ICOS.

Journal: Biomedicines

Article Title: Multi-Algorithm-Integrated Tertiary Lymphoid Structure Gene Signature for Immune Landscape Characterization and Prognosis in Colorectal Cancer Patients

doi: 10.3390/biomedicines12112644

Figure Lengend Snippet: Association between three TLS genes and the tumor microenvironment. ( A – D ) Annotations of all cell types in the CRC_EMTAB8107 dataset. Proportions and expression levels of ( E ) CCL2, ( F ) PDCD1, and ( G ) ICOS.

Article Snippet: Subsequently, CCL2 antibody IHC (GB11199, Servicebio, Wuhan, Hubei, China, 1:500) was applied to the TLS-positive samples to detect CCL2 expression levels in formalin-fixed paraffin-embedded (FFPE) specimens.

Techniques: Expressing

IHC and H&E staining characterized the presence of TLS and CCL2 expression in CRC and specifically within TLS regions. ( A ) H&E and CD20 IHC staining identified areas containing TLS. (B ) CCL2 expression in both TLS regions and adjacent tumor tissue. ( C ) Differences in the proportion of CCL2-positive areas between TLS regions and tumor tissue, with ** p < 0.01.

Journal: Biomedicines

Article Title: Multi-Algorithm-Integrated Tertiary Lymphoid Structure Gene Signature for Immune Landscape Characterization and Prognosis in Colorectal Cancer Patients

doi: 10.3390/biomedicines12112644

Figure Lengend Snippet: IHC and H&E staining characterized the presence of TLS and CCL2 expression in CRC and specifically within TLS regions. ( A ) H&E and CD20 IHC staining identified areas containing TLS. (B ) CCL2 expression in both TLS regions and adjacent tumor tissue. ( C ) Differences in the proportion of CCL2-positive areas between TLS regions and tumor tissue, with ** p < 0.01.

Article Snippet: Subsequently, CCL2 antibody IHC (GB11199, Servicebio, Wuhan, Hubei, China, 1:500) was applied to the TLS-positive samples to detect CCL2 expression levels in formalin-fixed paraffin-embedded (FFPE) specimens.

Techniques: Staining, Expressing, Immunohistochemistry